A letter sent by Dario Brando to George Joestar asking him to take care of his son Dio. It was then put into a box in the library of the Joestar Mansion and forgotten. Seven years later, Jonathan discovers it by accident, realizing that Dario was suffering the same symptoms as his currently sick father, George. This leads Jonathan to suspect Dio of having poisoned his own father and George as well. The address on the letter is changed from "225 Baker Street East, Liverpool" to "221B Baker Street, Liverpool" in the anime, making it a Sherlock Holmes reference.