A summary of Dio Brando's history and movesets in video games.
Miscellaneous Games
JoJo's Bizarre Adventure (SFC)
DIO will sporadically appear throughout the game to taunt the heroes and comment on what tarot cards have been chosen for them. He appears as the final boss of the game and, unlike the manga, the entire Joestar group is pitted against him (this includes Jotaro, Joseph, Polnareff, Kakyoin and the then-meant-to-be-deceased Avdol and Iggy.). He has an enormous 1,000 HP and primarily uses his Stand, The World, and its time stop ability to deal massive damage to the player characters. However, he has the glaring weakness of taking much more damage from attacks dealt by Jotaro if he has the Time Cap equipped.
Cult Jump (GB)
DIO appears as one of the ten characters from the JoJo's Bizarre Adventure franchise in the game.
GioGio's Bizarre Adventure (PS2)
Stardust Crusaders DIO appears in the game's opening during a scene where Giorno Giovanna looks at the picture of his father in his wallet, replicating the action taken in an early chapter of Vento Aureo.
Jump Super Stars (DS)
DIO is one of the many playable characters in Jump Super Stars. He can only be unlocked after clearing the game once.
He has three potential "battle panel" formations: a 4-block, a 5-block and a 6-block. All three have his characteristic "MUDAMUDA!" stream of punches as one special attack. His other special attack varies depending on the formation: With the 4-block, it's "The World, Circle of Knives", with the 5-block it's "The World, Road Roller" and with the 6-block, it's "The World, Time Stop". He also features in two "support panels" - the 2-block "Eye-Crushing Kick" and the 3-block "WRYAAAAA!" - and one "help panel" which renders the player's battle character immune from damage for a short period of time.
In terms of playing style, he is best for long-range attacks and uses a combination of punches and kicks. The World gives him a longer range than most characters, but at the cost of his speed. Dio is able to drains an opponent's health in close quarters (via sticking his fingers in their neck) and transfers it to himself. He can use "Space Ripper Stingy Eyes" as a regular attack, and has an aerial move which involves throwing knives.
Interestingly, he receives a power boost when his "battle panels" are placed beside those of Hao Asakura from Shaman King and/or Papillion from Busou Renkin. He is also able to perform a special tag-team attack with Sasuke Uchiha from Naruto called "Sharingan World", whereby DIO's homing daggers and Sasuke's shuriken slice through all enemies on the screen.
Jump Ultimate Stars (DS)
DIO returns in the Jump Super Stars' sequel, Jump Ultimate Stars and retains his previous playing style.
In the game, DIO can receive a power boost when his panel is placed beside Giorno (whom asks if DIO is one person with two bodies), Jonathan (who mocks him with his iconic line "MUDAMUDA!") and Taizo from Taizo Mote King Saga (whom asks if DIO remembers how many pairs of underwear he had worn to date, parodying Dio's classic line).
DIO also provides an ally boost to Freeza from Dragon Ball Z (he asks Freeza if he also seeks for immortality) and Dr. Mashirito from DR.Slump (he asks if Mashirito has his ideology; after winning comes world domination, a goal shared by both villains).
J-Stars Victory VS (PS3/PS4/PS Vita)
Aside from being mentioned by Jonathan only once in Story Mode, he does not appear in any form in the game. However, hidden data was found in the game for him and Jotaro as playable characters (alongside other Weekly Shonen Jump characters Yoh and Dai, from Shaman King and Dragon Quest: Dai no Daibōken respectively).