DIO (ＤＩＯ) as he appears in Stardust Crusaders was confirmed for Eyes of Heaven alongside Vanilla Ice. He appears as one of the 6 playable characters in the game's second demo.
He is both a Stand user and a Vampire. As a Vampire, he is able to steal opponents' health. All damage to him aside from Ripple-based will become "white damage", that can be healed over time, and inversely, DIO will take more damage from Ripple-based attacks. As a Stand user, DIO is one of many characters with wildly varied abilities that grant him uniqueness in battle.
Unlike All-Star Battle, DIO now sports default colors to match the Stardust Crusaders anime adaptation, most noticeably wearing a black shirt rather than his previously green one. Also, like the Part 3 anime, Takehito Koyasu reprises his role as DIO's voice actor from All-Star Battle.
In the semifinals, DIO is introduced in another team, paired with Jotaro Kujo. They defeat Mariah and Esidisi in the first round, but were eliminated by Jolyne Cujoh and Gyro Zeppeli in the second.
