Irene is a young woman bearing many striking similarities to Jolyne Cujoh as well as the star birthmark on her shoulder like the rest of the Joestar family. Unlike Jolyne, she has longer hair, and a softer outfit consisting of a light, turtle-necked sweater cut above the navel, patterned by several heart-shaped gaps or holes, bandeau bra with a spiderweb pattern and a crest.
In her brief introduction, Irene appears to be friendly and rather carefree, having offered a ride she shares with her boyfriend, Anakiss, to multiple strangers (Emporio and an unnamed woman); along with asking Anakiss to give a hitchhiker a ride despite his objections.
She shares an intimate relationship with her boyfriend and plans to get married under paternal approval; implying that contrary to Jolyne, she seems to have a better relationship with her father. Like Jolyne, she also adopts a motherly attitude to Emporio.
After the universe is reset due to Made in Heaven, Emporio Alnino defeats Pucci. The universe goes through another singularity and Emporio finds himself near a random gas station next to Green Dolphin Street Prison. There he meets people resembling his deceased friends, "Irene", her boyfriend Anakiss and an unnamed woman. Soon after Emporio misses the bus, Anakiss and Irene offer to give him a ride, assuring him that they aren't bad people. Irene tells Emporio that they were on their way to meet with Irene's father in order to ask for his approval of her and Anakiss' marriage. As they talk, the rain starts to fall and Irene comforts him, taking off her jacket to wrap it around a sobbing Emporio. Emporio notices the Joestar Birthmark on Irene's shoulder and tells her his name, with tears in his eyes. The group drives off, but not before Irene tells Anakiss to stop the car to give a ride to a hitchhiker walking on the side of the road.
Irene's voice and lines appear during the final text-based cut scenes of the Stone Ocean STORY MODE, sharing her voice actress, Miyuki Sawashiro, with Jolyne. The text consists of her conversation with Emporio where she introduces herself and offers him a ride (reenacting the the original story line).
Irene's outfit also appears as Jolyne's third alternate costume.
Like the previous game, Irene's lines are referenced in the game and her outfit appears as one of Jolyne's alternate costumes.
- Her name is the same as Irene Rapona from one of Araki's first works, Gorgeous Irene. In an interview, Araki states that there isn't any deep meaning or relationship between the two Irenes, and he was merely having fun.
- The names Irene and Anakiss form a word play when together: Irene (I lean) Anakiss (And I kiss)