The opening begins with Josuke Higashikata, Koichi Hirose, Okuyasu Nijimura, and Jotaro Kujo standing on a darkly lit stage illuminated by several multi-colored lights. The lights circle around the stage along with signs saying "JoJo" and "Part 4". It then flashes to colorful pop art portraits of the four of them with several stars and paint splatters in the background; Josuke is brushing his hair; Koichi has his arm reaching out; Okuyasu is holding a fist; Jotaro is holding the brim of his hat. It cuts back to the stage and the four of them point toward the ceiling as lights sparkle above them.
The scenery changes to different locations in Morioh as various silhouettes of all of Morioh's Stand Users instantaneously slide across the screen: Josuke and Koichi are shown in front of Morioh Station; Okuyasu, Keicho Nijimura, and Aya Tsuji are shown in front of Josuke's house in Jozenji; Former Tama, Akira Otoishi, Tamami Kobayashi, and Yoshihiro Kira are shown in front of Sun Mart; Masazo Kinoto and Anjuro Katagiri appear in front of a different shot of Morioh Station; Toshikazu Hazamada, Terunosuke Miyamoto, and Shizuka Joestar appear in front of the interior of Budogaoka High School; Yoshikage Kira, Mikitaka Hazekura, Joseph Joestar, and Yuya Fungami appear in front of the house at Boyoyoing Cape; Toyohiro Kanedaichi and Yukako Yamagishi are shown in front of the agricultural area; Rohan Kishibe, Shigekiyo Yangu, Bug-Eaten and Not Bug-Eaten are shown in front of Cafe Menphis; and lastly, Tonio Trussardi, Ken Oyanagi, and Jotaro Kujo appear in front of the Nijimura house.
Clouds fill the screen to reveal an aerial view of Japan with the season's logo as the camera zooms into Morioh, with the view quickly descending through clouds and towards the ground. The logo then shatters into many pieces and the camera zooms closer to Morioh Station.
Josuke walks on the street near Morioh Station surrounded by people and street signs, which reference different Stands throughout the season: there's a heart-shaped sign that says Crazy Diamond and "JOJO", a rectangular sign that says "DORARA!" below it, a diamond-shaped sign with Rohan's pen nib, a sign that says "ORA ORA ORA", a circular sign with Killer Queen's skull emblem, a diamond-shaped sign with Okuyasu's dollar symbol, and a sign with the Morioh logo. The scene zooms onto Josuke who turns to his left as the scene changes to focus on Koichi. Koichi, surrounded by green and purple backdrops, looks around nervously as it zooms out to reveal his shadow as Yukako Yamagishi gazing at him. The scene then switches to Okuyasu, who is knelt on the ground next to a soft-drink can. Once again, the scene zooms out to reveal Okuyasu's shadow as his brother, Keicho Nijimura, aiming the Bow and Arrow before morphing into their father. The shadows disappear as Okuyasu slowly stands up before kicking the can, leading into a transition of a photo of Josuke on the can.
The can slowly rotates clockwise before transitioning into Josuke walking down Jozenji street as disco lights illuminate him. Josuke strikes a pose, leading to Crazy Diamond appearing from his side as diamond-sparkles fill the scene. It transitions to Koichi running down a separate street with disco lights shining on the pavement, and he also strikes a pose. Colorful ripples then change the scenery to feature Okuyasu strolling down a sidewalk. He then leans backwards, which prompts The Hand to appear from his opposite side, mirroring the pose he's in. The scene zooms into them both before breaking apart to reveal Jotaro standing by himself. Jotaro then strikes his trademark pointing pose, with Star Platinum appearing from his side, mimicking him. Multiple colorful ripples dot across the scene resembling the appearance of stopped time, and the scenery suddenly pans out and upwards towards the sky.
The scene transitions into a map of Morioh, with Josuke, Koichi, Okuyasu, Hazamada, Tamami, Yukako, and Tonio all dancing along to the song. Lastly, Jotaro appears and smiles before Crazy Diamond's fist rises upward. He punches the background, destroying it, and reforming it as a plain background with a dotted pattern. The Hand then appears and uses its power to erase the background completely.
It flashes white to reveal Josuke, Okuyasu, and Koichi all standing in front of their high school. Josuke then turns around with a stoic expression on his face as the others turn with him. The scene pans out as the trio gaze upon the town of Morioh in its entirety from atop a hill. It focuses towards the sky itself before immediately cutting to Josuke, Okuyasu, Koichi, and Jotaro all striking a pose in front of a colorful background.
Altered Versions
Starting from the seventh episode onward, the opening includes Koichi's Stand, Echoes. The scene where Koichi used to be posing alone now includes Echoes ACT1 alongside him, which produces the word MYSTERY in green. The "MYSTERY" word then flies toward the screen, transitioning it to Okuyasu's scene. Later on, near the end of the opening, Echoes ACT1 appears on the new background that Crazy Diamond recreates, appearing like graffiti. It slightly moves upward and then The Hand erases the background like the original.
In DU Episode 8 and 9, "Crazy Noisy Bizarre Town (EDM Arrangement ver.)" is used instead of the original version. The opening credits were accordingly modified to reflect the usage.