This old and well-dressed man is the narrator of the story. Appearing as a bum during the game of poker bewteen Don Peckinpah and Mike Harper, the narrator had pointed out that both players had a four of a kind, queens. As the two outlaws drew their guns on each other, the bum used the distraction to throw a cocktail molotov and kill both of them. It is revealed at the end that he was a sheriff who got rich thanks to the bounties he's collected for killing Peckinpah and Harper.
A bomber that contacts and plays a sick game with the crew of the Dillinger. He's planted two bombs on the ship, the first destroys the escape pods and the second one, named "Virginia", will set off in about an hour. The bomber exhibits a playful and sadistic personality, treating his life-threatening plan like a game. When the crew disables the bomb, the bomber claims he's on the Dillinger and will now pursue Hiroshi. In the end, it's revealed that he was actually the ship's captain, Matt Jackson.
An associate of the Pinkerton Detectives. He was hired to get ahead of the outlaw and signal his position, but instead he tries to ambush the outlaw by faking having been hung. However, the outlaw sees that there are too few footprints on the ground for the hanging to have been genuine. His ambush fails, and the boy is later berated by the Detectives. When one man spits on him, he draws his gun in anger but a detective throws a knife in his chest, killing him.
A trio of detectives from the Pinkerton National Agency. They are expert trackers who hunt the outlaw. They relentlessly pursue him and grievously wound him, but the outlaw manages to kill two and injure the last one by ricocheting his bullets.
Koichi's uncle. He is a reporter working on the case of the murdered reporter and explains what has happened to B.T. and Koichi. He helps arresting the fat policeman when he listens to the latter's engineered confession.
A fat policeman who was among the first to discover the corpse of the reporter with Fuyuko unconscious nearby. However, it is discovered that he is corrupt cop working with the mafia and that the dead reporter had an incriminating photography of him. The fat policeman murdered the reporter by pushing the scissors further into the reporter's back and used Fuyuko as a scapegoat. However, B.T. forces him to reveal his true colors and he's arrested.
A reporter who has just been assassinated. Fuyuko claims that he tried to rape her and that she tried to defend herself, accidentally stabbing him in the back. It is discovered that the reporter had incriminating photographies and was maybe blackmailing the fat policeman, who then killed him. Koichi also discovers that the reporter had a photo of Fuyuko shoplifting, which may mean that she had tried to kill him.
B.T.'s grandmother. She is taking care of her grandson while B.T.'s parents are away. She seems to work as a detective as she is shown having investigated a corrupt museum director, and is adept at manipulation as she manipulated B.T. into creating a good diversion for her collaborator.
A violent lunatic living in the countryside. He disguises himself as some sort of military leader and director of a camp, donning an SS outfit, a fake mustache, reminiscent of Adolf Hitler, and a military cap with a Swastika symbol, not unlike the one used by the Nazis. The Leader kills small animals for kicks and attempts to play sick games with B.T. and Koichi. Thankfully, B.T. manages to defeat him.
Manabu's family. They typically try to make it look like their victim is hurting them, using their guilt to invade their home, steal their possessions and prevent the victims from calling the police. Eventually, they'll steal everything from the victims. Their latest victims were the Mugikari family but B.T. got rid of them by poisoning them with cyanamide, a chemical that gives stomach cramps when mixed with alcohol. By making them believe they just ate sashimi made out of the poisonous fugu fish, B.T. forces them to get carried to the hospital.
Six young men and women working for the Outlaw Guys company. Their job is to contact and scam old people into investing their savings in various business ventures or properties that are all set up to fail so that the victims lose their money and any possession they've used as collateral. They even use fake houses to trick their victims into buying a property. They disdain the old people they scam, but are deathly afraid of their own boss.
Three masked men among the higher ranking members of the Dress Organization and who act as Dr. Kasuminome's supervisors. Their identity is top secret so not even Kasuminome is allowed to see their faces or know their names. Kasuminome explains to them the mechanisms of the biological weapon "Baoh" and they seem truly impressed by the weapon.
A test subject dog who becomes the first organism to have Baoh implanted within, to demonstrate Baoh's power to Dr. Kasuminome's supervisors. Kasuminome keeps the dog in a water tank to ensure Baoh goes into a state of apparent death via asphyxiation. When going against a tiger in an experiment, the dog starts cowering around. The tiger rips off the dog's eye, seemingly killing the latter. However, the dog stands up, transforms, and swiftly kills the tiger, melting its corpse afterwards. Then the dog tries going for Kasuminome and his men, charging at and cracking the containment glass, and prompting Kasuminome to order the dog to be shot at with lasers. The dog eventually dies after being shot in the eye, and its Baoh parasite comes out of it. Kasuminome then orders the dog's corpse to be incinerated.
The dog dies in a different way in the OVA, where it is shot in the brain by Dordo after attempting to attack the latter.
An ordinary tobacco vendor. She's asked by one of the Dress Soldiers if she's seen Ikuro Hashizawa and says no, not bothering to look up. In response, the soldier slams her head against the desk, ordering her to look at a picture of Ikuro. She gives the soldier the information he needs.
Martin's master. He is a cruel beastmaster and agent of the Dress Organization. He's sent after Ikuro Hashizawa and uses his mutated mandrill Martin to fight him. Martin's master himself wields a whip to command Martin and he also uses it to whip Sumire. This angers Baoh who shoots his flaming hair at the beastmaster. Martin's master thus dies a painful death.
Ikuro's Parents. The Hashizawa family's car crashed against a cliff when they had to swerve around two speeding cars. Although The mother and father could have survived their injuries, Dr. Kasuminome had them injected with poison so that no one would search for Ikuro, whom he wanted as a guinea pig.
A doctor and a low-ranking member of the Dress Organization. When the Hashizawas are brought to him, he contacts Dr. Kasuminome, informing him that a guinea pig (the young Ikuro) is available. Despite his protests and the assurance that he could save all three, Kasuminome has the parents killed and the doctor is cowed into staying silent.
Aroma Bats (芳香蝙蝠,Aroma Batto) are special bats created by Dr. Kasuminome. They can be controlled by ultrasonic waves, which Dordo utilizes. The special aroma emanated by these bats can paralyze the Baoh parasite's Tactile Sensor. Rokusuke shoots all of them down.
Sap Spiders (液クモ,Eki Kumo) are species of mutated giant spiders that Dr. Kasuminome sics on Ikuro Hashizawa. They are bigger than a grown man and can resist the digestive enzymes that are used to flood one corridor. They can deploy a sharp tongue from their mouths to suck the liquefied bodies of their victims.
The Skookum Tribe (スクークム族,Sukūkumu-zoku) is a fictional, non-perished tribe of Native Americans. Its last known member is Walken. One of their traditions is carving a symbol of blood as mannerisms of respect through death to a true warrior.
Irene's butler. He served her family and took care of her after the death of Irene's parents. He warns the tailor's son about Irene and to never lie to her, otherwise the butler will kill him. He seems to have some combat abilities too as he's able to split a flying fly in two. He was later killed by an assassin sent to kill Irene. Before dying, he tells a squirrel to tell Irene about his death and the assassin coming for her.
Irene Rapona's late father. He was an assassin in the mafia. His boss, his wife and himself were killed but his daughter Irene survived. It is said that Irene inherited her hypnosis abilities from him.
A tailor who had the bad luck to attract the crime lord Lauper's eye. She ordered him to make her a dress in a night but he needed to order more material. Lauper got offended and beat him, but he showed defiance and was killed for it.
A squirrel that Irene's Butler was carrying with him at the moment of his death. The butler tells it to go and warn Irene to flee, which it seemingly does offscreen. It then tags along Irene and is often seen on her shoulder.
A drug dealer whom Irene meets in a bar. He has a distinctive appearance with his large flattop and his heart-shaped eyepatch. He offers her a bag of drug, and gets angry when she takes it without paying. When she says she has no money, the dealer offers her to become a "movie star"; Irene accepts without thinking but Michael intervenes and elbows him in the face. Later, he helps a Female Assassin finding Irene by identifying the girl on a photo.
A female assassin sent by the enemies of Irene Rapona to kill her. She kills Irene's butler and later finds Irene. However, she is swiftly killed by Irene. She has a special tattoo from which a spectral arm armed with a knife can appear, and she also commands some deadly ants to attack her foes.
Two students who have just ended their friendship with each other. The green-haired student states he and the other student are through as friends. The blue-haired student asserts that he hopes he never has to see him again. Josuke Higashikata sees the ex-friends and tries to fix their friendship with his Crazy Diamond. Instead, they are both killed by its punch barrage.
A translator from the Speedwagon Foundation who worked on restoring and deciphering DIO's diary. He confesses that, although the foundation's goal was to find the information that may help them free Jotaro Kujo from his current comatose state, he was mainly motivated by his fascination with DIO.
The translator also comments on the difficulty of the foundation's request. The diary was not fully restored after being burned by Jotaro Kujo, even with the help of Josuke Higashikata, and its contents were hard to understand even for a specialist like him. Therefore, the translation may include various errors.
A boy living in Cairo. He's posted himself in front of the area affected by Tenore Sax and tries to take advantage of Hol Horse when the latter cannot go through by telling him to pay so that he can guide him. Hol Horse gets rid of the kid by breaking some flower pots with his Stand and framing the boy, forcing him to flee from the wrath of the man who was watering the plants.
A gang of thugs who are acquainted with Yuya Fungami. The leader has no care for others and parks horizontally in the middle of the road, blocking the traffic. He complains about Yuya getting too cocky when an elderly lady walks up to him and asks if he can move his car. The thug furiously tosses his phone at the old lady, asserting that she made him miss part of an important call. However, Ryoko Kakyoin deflects the phone away with her bag, causing it to break when it hits the ground. The thug first assumes Ryoko might be one of Yuya's girlfriends but the thug's girlfriend comments that Yuya only likes pretty girls.
The thug insults Ryoko's dangling hair, claiming Yuya wouldn't give the time of day to someone like that. Ryoko then smirks and asks if he was talking to the boy behind her, pointing out Josuke. Instantly, Josuke rapidly punches away the thug's car with Crazy Diamond and reshapes the roof of their car into a cage, trapping them while he continues kicking their car.
The father of Oingo and Boingo. He seems to be abusive as Oingo and Boingo are afraid of him. After seeing Boingo's comic book, he sells it for drinking money, but the book ends up coming back to Boingo later.