Since most of the game's animation, effects and attacks were reused from All-Star Battle, Josuke's repertoire of abilities remains mostly the same, though with some new additions to aid him in battle.
As a Stand user, Josuke is one of many characters with wildly varied abilities that grant him uniqueness in battle.
Style Action - Crazy Diamond: Josuke summons Crazy Diamond to deliver a punch that is able to generate a number of different effects.
Under normal circumstances, Crazy Diamond's punch hits the ground to dig it up, reforming it into a floating barrier that can block projectiles and absorb sufficient damage before breaking, while also leaving a small, visible spot of excavated earth that Josuke can return to via "As you were!"
If Crazy Diamond's punch hits an opponent, the barrier forms around them, trapping them inside and paralyzing them. This leaves them vulnerable to more attacks. Quickly moving the movement stick around can shorten the time bound. This will also leave the spot of dug-up earth.
If Crazy Diamond's punch hits Josuke's partner, they will recover a small amount of health. No barrier or "As you were!" spot is created.
Trait - I'll heal you, no problem.: While standing near the spot of a broken stage object, Crazy Diamond can repair it and have it available again immediately, with no limit to the number of times this can be done.
As you were!: If the excavated spot of earth created by his Style Action is present, Josuke, using a piece of rubble he took from the floor, quickly flies toward the spot by fixing the piece. This move can be used from anywhere on the stage, and is excellent for evasion or covering large distances. If the floating barrier is present, Josuke will restore its durability to maximum upon reaching his destination. If there is any obstacle such as a wall in his path, Josuke will immediately stop, canceling the ability.
You're in my range...: Crazy Diamond flies forward and punches. If it connects with an opponent, Crazy Diamond will unleash a barrage of 24 punches, sending them flying on the final hit.
DORA!!: Crazy Diamond performs a chop that sends a vertical "wave" of blood a short distance forward as a projectile. If the blood hits an opponent, even if they block it, they will take a small amount of damage and become Bloody for a short period of time, and susceptible to a follow-up. Of note is that Josuke cannot perform this skill again on a different target if one of the opponents is actively Bloody.
Meet my homing missile!: Crazy Diamond flicks a shard of broken glass that targets the Bloody opponent with very strong homing capabilities, dealing substantial damage and leaving them crumpling and open to further attack. Even if it is blocked, the broken glass will erratically boomerang back through its target multiple times, dealing immense damage to their guard. This skill has a separate, longer cooldown than "DORA!!".
I'll just have to pummel you from here!/I'm a real Deadeye Dick!: The attack unleashed is dependent on whether or not the activating button is held. If pressed, Crazy Diamond flicks two ball bearings forward as projectiles, with their trajectories diverging and accuracy weakening the farther they travel. If held, Crazy Diamond flicks a rifle bullet as a projectile with high speed and accuracy, but extends the skill's cooldown.
EX - You're in my range...: Josuke is invincible to projectiles for the duration of the attack.
EX - I'm a real Deadeye Dick!: The rifle bullet is immediately ready and gains a damage boost, and Josuke is invincible to projectiles for the duration of the attack.
Trait - Watch your mouth: When Josuke's health drops below 20% (or a little below his last health gauge), he will begin to fix his pompadour while the opponent that last hit him will stop to insult it, temporarily sending him into a blind rage as his hair spikes upward. Josuke's damage receives an incredible boost, his Homing Dash speed is increased, he gains super armor (or unflinching to enemy attacks short of grabs and DHA), and unlimited stamina for a short period of time. In exchange, he takes more damage, cannot guard, and his Homing Dash loses its homing capabilities.
Dual Heat Attacks
Solo - I went ahead and healed your sorry ass...: Identical to its performance in All-Star Battle, though executes faster for the sake of brevity.
With Okuyasu - Hey, are we gonna do this, or what?: Okuyasu has The Hand bring their opponent closer by eliminating a wide space in front of them, leaving them staggered. After Josuke and Okuyasu briefly and excitedly share comments, Crazy Diamond and The Hand pummel their target with a dual Stand barrage followed by a joint uppercut that sends them high into the air.
With Koichi - Dorara Rush ×2: Koichi has Echoes Act 2 place SFX of Crazy Diamond's Stand Cry ("DORARARA!!") directly under the opponent's feet. He then prompts Josuke to attack, watching as he jumps high into the air until he is directly above the opponent. Crazy Diamond then punches the opponent from above as the SFX activates, creating artificially-made fists to attack from below, hitting them from both sides.
With Rohan - I really hate this guy...: Rohan uses Heaven's Door to write within the opponent "My body will fly forwards at 70 km/hr." as he begins to tell Josuke something; revealing soon after that he hates him. The opponent is sent flying at Josuke in an attempt to cause a collision, forcing him to use Crazy Diamond to pummel them away.
With Old Joseph - Gotta be cool, at my age.: Josuke & Crazy Diamond pummel the target, with the final punch sending them high into the air. Old Joseph then comes from behind Josuke and uses Hermit Purple to bind the airborne opponent. Yanking on his Stand and pulling them in, Old Joseph then charges and delivers a forceful Ripple-infused downward chop to blast the target away once more, leaving Josuke impressed.
Costumes & Tints
Normal
Special A
Special B
Special C
Special D
Special E
Special F
JoJolities
DORARA!!: Josuke must use his Style Action 3 times. (200 Points)
As you were!: Josuke must use "As you were!" 3 times. (200 Points)
Meet my homing missile!: Josuke must connect "Meet my homing missile!" 3 times. (300 Points)
I'm not done kicking your ass!: Josuke must achieve a collective hit count of 300. (500 Points)
This feels awesome!: Josuke must Retire an opponent with a Dual Heat Attack. (800 Points)
Misc.
He temporarily serves as the main protagonist of the story, along with Jonathan when the story enters the Steel Ball Run universe. He encounters his counterpart and fight a corrupted Joshu Higashikata. After defeating Joshu, he is attacked by Yoshikage Kira and his Kosaku Kawajiri counterpart.
(The information below derives from a Video Game not written by Araki. As such, it may not be considered canon.)
Giorno Giovanna: Giorno steals Josuke's wallet, causing Josuke to tremble with anger upon discovering it's been stolen. Giorno notices that it "only has 12 yen," a reference to the "Shigechi's Harvest" arc. After a match between the two, Giorno notes that his Stand ability and Josuke's are similar. When paired together, the two consider themselves a great tag team and note it is like they can read each other's moves.
Jolyne Cujoh: Josuke is surprised Jolyne is Jotaro's daughter. The two mock each other because they both look like delinquents.
Insults for Josuke's Hair
This is a list of the various insults each character gives to Josuke's hair in Eyes of Heaven. Upon having his hair insulted, Josuke will reply with "I won't forgive you for insulting it!" (けなすやつあゆるさねえ~~~, Kenasu yatsu a yurasane~~~). Note that characters not listed here reuses their quotes from All-Star Battle (in Part 4 Jotaro's case, he reuses his Part 3 self's line from ASB), though they are re-recorded with their respective, new voice actors in cases of the Part 3 cast (except DIO) and Pucci. Japanese translations taken from here, except unused voice clips.
Quotes
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I've seen a lot of things in my life up until now! However, I've never seen such a strange head like that! (おれは!今までの人生でいるんな物を見て来だ!だが、こんなヘンな頭は見たこともねえッ!!)