Captain Tennille (キャプテン・テニール,Kyaputen Tenīru) was introduced to the Joestar group by the Speedwagon Foundation to aid in their trip to reach DIO. His background was checked to make sure he wasn't a Stand user, so everyone could trust him. The impostor Captain Tennille killed him in Hong Kong and threw his body into the bottom of the sea, taking his place. Though the reader is never shown the true Captain Tennille, he was presumably almost identical to the impostor, since his men, who had worked for ten years on the same ship, did not even suspect the replacement.
Prince (プリンス,Purinsu), Michael (マイケル,Maikeru), and Lionel (ライオネル,Raioneru) are three of the five chickens Muhammad Avdol raised on the secluded island he lived on after faking his death. Disguised as his father so no one would come after him as he recovered from Emperor's bullet wound, Avdol fed the chickens so they would grow fat and tasty, but didn't like it when they ate too fast. When Avdol rejoined the Joestar group, he left the chickens on the island with no one to take care of them.
Caravan Serai (キャラバン・サライ,Kyaraban Sarai) was a blacksmith who created the sword that the Stand Anubis was bound to, and thus was its first user five centuries before Stardust Crusaders. When Serai died, Anubis did not die with him, but rather, without a user, began to search for users on its own.
D'Arby won the soul of Mrs. Moor through gambling, leading her husband to gamble with him on September 22, 1984, around 11:15 PM in California, to win her soul back. Stephen was defeated and had his soul taken too. His father Garie Moor (ゲイリー・ムーア,Geirī Mūa, Daddy Moor in the anime) tried to win his soul back, but was also beaten. Stephen may have almost won the gamble, as D'Arby remembered the date, place and even what he said before losing (the same words Muhammad Avdol said when Jean Pierre Polnareff was beaten). The Moors' souls were transported back to their bodies after Jotaro Kujo defeated D'Arby.
Christian Vander (クリスチャン・ヴァンデ,Kurisuchan Vande) was one of the many victims of Daniel J. D'Arby, losing his soul in a gamble to him some unspecified time after the Moor Family. His soul was returned to his body after D'Arby was defeated by Jotaro Kujo.
Chibi and Buchi (チビとブチ) are two dogs who try to attack Iggy as he faces DIO's Mansion. Iggy intimidates them with one look. Stumbling at the gate of the mansion, the dogs see Pet Shop's shadow and, assuming that the bird will be easy prey, crawl under the gate. Pet Shop kills them both by impaling their heads into an ice pike, and Pet Shop plucks their eyes out as a meal.
Dr. Elliot (エリオット医師,Eriotto-ishi) was a mass murderer who killed eight of his patients. Telence considered the stories of the murders interesting. His soul was put into a doll wearing doctor accessories made by Telence himself.
Tatsuhiko (タツヒコ) was a Japanese boy with an IQ of 190. According to Telence, he was a skilled video game player and Telence had a tough time trying to win his soul. In his doll form he called for his mother.
Al Yankovic (アル・ヤンコビック,Aru Yankobiku) is a singer that Jotaro mentions when testing Joseph to see if it was actually him or DIO in disguise. He asks who sang the parody of the song "Beat It", named "Eat It". Joseph correctly answers that it was Al and is saved from a beating from Jotaro.