Captain Tennille (キャプテン・テニール, Kyaputen Tenīru) was introduced to the Joestar group by the Speedwagon Foundation to aid in their trip to reach DIO. His background was checked to make sure he wasn't a Stand user, so everyone could trust him. The impostor Captain Tennille killed him in Hong Kong and threw his body into the bottom of the sea, taking his place. Though the reader is never shown the true Captain Tennille, he was presumably almost identical to the impostor, since his men, who had worked for ten years on the same ship, did not even suspect the replacement.
Sophie (ソフィー, Sofī) was a friend of Sherry Polnareff. She was with Sherry the day J. Geil attacked them. Surviving the attack, she later told Polnareff about the man who killed his sister, describing details like how some invisible shield protected J. Geil from the rain, how it felt as if the wind slashed her chest, and that the man had two right hands. Her testimony was met with incredulity, but Polnareff was able to surmise thanks to her testimony that Sherry was killed by a Stand user.
Sophie is unnamed in both the manga and the anime adaptation, and was named in the CD Drama adaptation of Stardust Crusaders.
Prince (プリンス, Purinsu), Michael (マイケル, Maikeru), and Lionel (ライオネル, Raioneru) are three of the five chickens Muhammad Avdol raised on the secluded island he lived on after faking his death. Disguised as his father so no one would come after him as he recovered from Emperor's bullet wound, Avdol fed the chickens so they would grow fat and tasty, but didn't like it when they ate too fast. When Avdol rejoined the Joestar group, he left the chickens on the island with no one to take care of them.
Fake Sherry is an imitation created by the stand Judgment when Polnareff asks it to revive his dead sister after the Stand offers to grant him a wish. The Stand manifests an imitation that looks and sounds exactly like her, including having her memories. It suddenly attacks Polnareff when he lets his guard down and gets close enough. The figure is ultimately destroyed when Judgement throws it at Magician's Red as a diversionary tactic.
In the anime, however, this imitation is still alive. Polnareff catches the figure, and, accepting the death of his sister, impales the clay figure, killing it for good.
Fake Avdol is the second imitation created by Judgment. It nearly perfectly resembles Avdol and seems to be a sadistic entity. When Polnareff begs for fake Sherry to vanish, Judgement taunts him and celebrates victory as he reveals Polnareff's 3rd wish, the return of the deceased Avdol. It attacks Polnareff alongside Sherry but gets dispatched when the real Muhammad Avdol ambushes them with Magician's Red.
Caravan Serai (キャラバン・サライ, Kyaraban Sarai) was a blacksmith who created the sword that the Stand Anubis was bound to, and thus was its first user five centuries before Stardust Crusaders. When Serai died, Anubis did not die with him, but rather, without a user, began to search for users on its own. Serai's sword would eventually find itself in a museum warehouse where it was found and recruited by DIO.
D'Arby won the soul of Stephen Moor (S・ムーア, Sutībun Mūa) through gambling on September 22, 1984, around 11:15 PM in California. Stephen may have almost won the gamble, as D'Arby remembered the date, place, and even what he said before losing (the same words Muhammad Avdol said when Jean Pierre Polnareff was beaten). It's unclear in what order that his wife Mrs. Moor and his father, Garie Moor (ゲイリー・ムーア, Geirī Mūa, Daddy Moor in the anime), had their souls taken by D'Arby after being beaten by him as well. The Moors' souls were freed after Jotaro Kujo defeated D'Arby.
Christian Vander (クリスチャン・ヴァンデ, Kurisuchan Vande) was one of the many victims of Daniel J. D'Arby, losing his soul in a gamble to him some unspecified time after the Moor Family. His soul was returned to his body after D'Arby was defeated by Jotaro Kujo.
Chibi (チビ, Chibi) and Buchi (ブチ, Buchi) are two dogs who try to attack Iggy as he faces DIO's Mansion. Iggy intimidates them with one look. Stumbling at the gate of the mansion, the dogs see Pet Shop's shadow and, assuming that the bird will be easy prey, crawl under the gate. Pet Shop kills them both by impaling their heads into an ice pike, and Pet Shop plucks their eyes out as a meal.
Dr. Elliot (エリオット医師, Eriotto-ishi) was a mass murderer who killed eight of his patients. Telence considered the stories of the murders interesting. His soul was put into a doll wearing doctor accessories made by Telence himself.
Tatsuhiko (タツヒコ) was a Japanese boy with an IQ of 190. According to Telence, he was a skilled video game player and Telence had a tough time trying to win his soul. In his doll form he called for his mother.
Al Yankovic (アル・ヤンコビック, Aru Yankobiku) is a singer that Jotaro mentions when testing Joseph to see if it was actually him or DIO in disguise. He asks who sang the parody of the song "Beat It", named "Eat It". Joseph correctly answers that it was Al and is saved from a beating from Jotaro.