Background
When she was younger, she went to collect nuts in the woods with her brother. Attacked by a grizzly bear, they hid in a rock crevice. Injured and scared, Hot Pants pushed her brother out of the crevice - saving herself at the price of his life. The people in her hometown were very kind to her and her parents after the "tragic accident", but Hot Pants became unable to bear the burden of the truth surrounding her brother's death. She left her home and joined a convent in Italy, becoming a nun in the hopes of earning forgiveness from God and her brother.
Steel Ball Run
Fourth Stage
Hot Pants first appears at the beginning of the race's Fourth Stage, having finished first in the Third Stage. Soon she encounters Johnny and Gyro, accusing them of having killed a cow she had been specially grazing on the course. She ends up attacking them using Cream Starter, preventing them from attacking by covering their hands and faces with her Stand's "flesh spray." Johnny convinces her that they weren't responsible for the cow's death, and she recalls the effect of her Stand. She refuses to apologize for blaming them as they had eaten meat from the carcass, then gallops away to hunt down the real culprit.
However, they meet again sooner than expected as all three become trapped in the orchard surrounding Ringo Roadagain's cabin. They witness the confrontation between him and Gaucho and end up deciding they will have to kill Ringo in order to continue. While Johnny and Ringo talk, Hot Pants attacks the latter with Cream Starter, slicing off his hand. Unfortunately, he rewinds time and reverts the damage, sidestepping the attack when it happens a second time and shooting Hot Pants' hand. He and Johnny then engage in a gunfight, which Johnny seemingly loses, and Ringo incapacitates Hot Pants by shooting her as well. After Gyro defeats Ringo, he and Johnny use Cream Starter to fill their and Hot Pants' wounds. Johnny then inadvertently discovers that the unconscious Hot Pants is a woman when he checks her chest to see if she is still breathing.
After the Race
Hot Pants drops out of the race in its sixth stage to further pursue the Corpse Parts. She meets Lucy Steel, who wants to give the corpse parts to Gyro and Johnny after their altercation with Blackmore. She gives Lucy some meat spray from Cream Starter to change her appearance in order to infiltrate Valentine's compound. She tells Lucy how to bring the corpse parts together using one of the eyes of the corpse. After Lucy is found out and ends up killing Scarlet, Hot Pants enters the compound to help her and fights off Mike O. and his Stand Tubular Bells. After spraying him with Cream Starter, she causes Mike O. to accidentally kill himself by filling himself with air. Before escaping, she switches Lucy's face with Scarlet's to make it appear that Lucy was the one who died.
Later, she is seen by Gyro and Johnny speeding towards a church. When Gyro goes inside the church, she disguises herself as a nun (Gyro does not know that she is a woman at this point). Johnny comes into the church as well, and when Gyro leaves to find Hot Pants, Johnny reveals to Hot Pants that he knows her true gender. For the first time, she breaks down into tears as she tells Johnny that her Corpse Parts were taken by someone. At that moment, her deceased brother appears on her body due to Civil War. She says that the guilt has followed her even as she collected Corpse Parts, even when she became a nun in her hometown. She tells Johnny to run as the memory of her brother's death nearly consumes her. After Axl Ro is killed, she is absolved of her sins (at least physically) and is left by Gyro & Johnny.
Death
Hot Pants, after trying to suffocate Diego, teams up with him to take the corpse from the President. The duo, after catching up to the train that Valentine is on, fight off the many Funny Valentine clones brought forth by D4C. After Diego's death, Hot Pants boards the train car that is holding Lucy Steel. She is marveled over the mosaic of the Corpse Parts on Lucy's body. However, Lucy's Stand, Ticket to Ride, finally takes form and the President manipulates the Stand for his own use. Hot Pants is the first to fall victim to the Stand's ability when D4C has multiple insects and a window frame be absorbed under her skin, puncturing her heart and nearly killing her. This causes Hot Pants to fly out of the moving train and tumble onto the ground, where she perishes.