George Joestar III (ジョージ・ジョースターⅢ世,Jōji Jōsutā Sansei) is Johnny and Rina's first son and their second and last child, brother of his unnamed sister. He was born in 1898. When George's mother was on the verge of death, Johnny used the corpse to remove her disease, but the equivalent exchange only caused the illness to move onto George. The boy was saved when Johnny transferred the illness to himself with Tusk ACT 4, but the transfer also caused him to fall off his horse onto a bed of leaves with Les Feuilles, leading to a large rock being displaced onto his head shortly after. George was later found by agents from the United States and his mother, Rina.
As an adult, he is the father of two daughters and two sons, one of them being Joseph Joestar.
Joseph Joestar is the second son of George III and Elizabeth as well as their fourth and last child. He was born in 1924 and is the husband of Suzi Q and father of Holy Joestar-Kira. Likely the SBR universe counterpart of Joseph Joestar.
Tomoko Higashikata is the mother of Norisuke Higashikata IV (whose birth name was Josuke Higashikata). She reassured him when he asked questions about the family's curse, and died when the Rock Disease was transferred from him to her.
A taxi driver named Muneyuki who shows disappointment after Karera Sakunami tells him to drive her and Josuke to Kira's apartment, because of the very short distance. Angered by his reaction, Karera throws a tantrum and the cab driver tries to kick her out of his cab, pointing out that there are cameras. She then settles down and coldly blames him for closing the door on her hair, saying she won't tell as long as he cooperates.
Atsunori Iwakiri (岩切 厚徳,Iwakiri Atsunori) is a star pitcher for the Seiten Birdies baseball team, nicknamed "God shoulder A-kun" by the crowd. In 2009 he had a salary of 300 million yen. However, in 2010 he damaged his shoulder with small hope of recovery and went on the disabled list just before he was going to be accepted into the major leagues. Desperate, he bought a Locacaca fruit for 200 million yen from Aisho Dainenjiyama to heal his shoulder. He made a full recovery in exchange for his teeth and lower jaw, which turned to stone.
Zaihei Nigatake (苦竹 財平,Nigatake Zaihei) is a man who tricked Joshu Higashikata into being cursed by the Milagro Man's money, which he received by stealing from a cursed man in Europe who was pretending to be homeless. Once confronted, he explains the curse to Joshu and gives him advice on how to get rid of it. Joshu decides to return the curse to Zaihei using his Stand, and walks away afterwards, leaving Zaihei overwhelmed by the Milagro Man's money.
Rena Nanami (菜々美 レナ,Nanami Rena) is a famous idol with many fans (especially male ones). Joshu is particularly infatuated with her and pressures his father to give him money to go to one of her conventions. After meeting her, he states it was an awesome experience and that her fingers are so smooth; however, her fingers and cuteness take a backseat to Yasuho's.
Miko (ミコ,Miko) was an unseen girl whom Yasuho knew when she was younger. It is implied that the two of them were friends. One day, Yasuho was supposed to come to Miko's birthday party, but completely forgot about it because she was distracted by her new hair clip. The moment she realized, she received multiple insulting texts from the many party attendants.
Mina (ミナ) is a classmate of Tsurugi Higashikata. She and her friends would taunt and harass Tsurugi because of the rumors of his grandmother burying a child's corpse. Mina teases Tsurugi by telling him to to ask Kaato how it was possible for her to bury the corpse without it rotting. Tsurugi glares at them and they mock his eyes. Mina then pushes Tsurugi further by flicking his forehead and saying it was his father who actually killed the boy. A frog then hops on Mina's foot and she falls into the nearby river.
After the incident, Mina's body is found collapsed in between the iron rails of the school gate. Her underwear was stained red from the injury. Although she was merely unconscious and wasn't dead, she wasn't responding. One of Mina's friends blames it on Tsurugi, saying she saw him make the gates move.
Later, the school principal says Mina's injuries were just minor light scratches and she's recovering fine in the hospital.
It is implied that the gates closing in on Mina was actually a calamity caused by the Head Doctor, as Mitsuba saw him standing near the gates moments after the incident on the school monitor.
For unknown reasons in Chapter 91, Mitsuba calls Mina "Miwa" (ミワ,Miwa).
Taoka (田岡) is a reporter from the science journal "Pangea Land" (パンゲア ランド). He attends Satoru Akefu's lecture on regenerative medicine at the TG University Hospital. Josuke sends him a package containing footage of a bedridden Holy Joestar-Kira and the secret laboratory, plus a sample of the Locacaca that he checks by opening the jar, accidentally exposing himself to the fruit. Taoka confronts Akefu over this and accuses the head doctor of conducting illegal human trials. He intends to legally pursue the doctor and shut down the hospital but this triggers Akefu's ability. Because of the fruit and Akefu's ability, Taoka undergoes a fatal Equivalent Exchange, curing his pimples in exchange for his vertebrae. His neck bone crumbles, causing his head to twist and tilt, thus suffering a horrible death. However, he's served his purpose of alerting Akefu and making him go to the laboratory where Josuke awaits the head doctor.
Satoru Akefu (明負 悟,Akefu Satoru) is the identity stolen by Tooru's Stand, Wonder of U. A 79 year-old Japanese man living in the Ibaraki Prefecture, Akefu was a former member of the Society of Obstetrics and Gynecology, and a graduate of the TG University School of Medicine. Tooru learns of Akefu's existence during his first encounter with Yasuho 10 years before the events of JoJolion, after asking her to look up elderly Japanese men who live in nursing homes, once had a license to practice medicine, and are cut off from their family. Yasuho states that while there aren't many individuals fitting that description, Akefu is the closest one to their location. Tooru notes that Akefu's identity would be perfect to impersonate, and quickly leaves afterwards.