The opening scene shows Jonathan and the younger Joseph, before showing Jotaro, initially turning his back on the screen, then turning around, pointing at the screen and summoning Star Platinum, who then unleashes his blazing fists. The Katakana characters "Jo" can be seen popping out at the screen as Star Platinum punches repeatedly, before the logo is seen entirely.
The next scene after the logo shows 5 shooting stars zooming into the skyline, before crashing into the ground. A 6th shooting star is seen at the far right, speculated to represent Iggy, who is yet to be introduced.
After the shooting stars come down crashing, the scene zooms into the darkness of DIO's mansion, which then zooms into his shady figure, emphasizing his Joestar birthmark courtesy of Jonathan's lower body. The scene changes as DIO swipes his hands onto the screen, zooming out from the eyes of Star Platinum, Jotaro keenly observing a Polaroid picture of Dio along with Avdol and Joseph.
The scene changes as the party quintet (with Kakyoin and Polnareff) walks, reflecting the many places they'll be traveling. A figure speculated to be Hol Horse is seen briefly.
The same quintet is seen riding a camel at the desert, then in the night sky, Polnareff jumping excitedly, and then all of them converging in a firecamp.
A montage of Holly and other characters is seen descending, until showing Jotaro, crouching, who then stands up sharply, with his Stand emerging from him.
A brief sketchy view of DIO is seen, that zooms into his eyes.
The quintet then demonstrates their Stands, starting with Joseph, Avdol, Kakyoin, and Polnareff. Jotaro then travels into a 3D-rendered scene with many writings, Katakana and English characters revolving around, namely ORA, JoJo, LUCKY LAND and others. As Jotaro zooms in, Joseph emerges from the other end, using his Stand to travel, while Avdol, Polnareff and Kakyoin demonstrates their Stand one after the other.
A brief view of Jonathan closing his eyes (presumably from his death scene at the burning boat), Joseph, and Jotaro is then seen, while the song chants "JOJO!" 3 times, presumably counting the 3.
The next scene has the tarots revolve around, with Star Platinum and Jotaro emerging, and a heavily obscured DIO. The scene changes with Jotaro posing, then clutching his Gakuran hat, and sharply tweaking it, with Star Platinum emerging from him. The final scene shows Star Platinum punching a mirror that briefly reflects Dio. Afterwards, Jotaro walks towards it while Star Platinum repeatedly punching the glass pane until eventually smashing it, the perspective actively changing. Finally, the fist of Star Platinum emerges from the cracked and shattered glass.
The intro has obvious 3DCG elements in use that is comparable to Sono Chi no Sadame, especially in the last parts, where the animation of Star Platinum and Jotaro is remarkably near-realistic.
Let its fists fly this hallowed vision, STAND PROUD!
封印(と)じられてた「時」に
Tojirare teta toki ni
陽の光 浴びせるのは誰だ
Hi no nikari abiseru no wa dereda
未来 遺す希望
Mirai nokosu kibou
流れる星の クルセイダース
Nagareru boshi no Kuruseidasu
賭けるのは命 護るべき愛と
Kakeru no wa inochi mamorubeki ai to
TAROT(カード)は配られた
Kaado wa kuba rareta
Stand up! Stand up! Stand up!
Stand up! Stand up! Stand up!
(Stand up! Stand up! Stand up!)
立ちはだかる
Tachihadakaru
All right now All right now All right now
All right now All right now All right now
All right now All right now All right now
敵を倒し
Teki wo taoshi
Break you down Break you down Break you down
Break you down Break you down Break you down
(Breakdown! Breakdown! Breakdown!)
道を拓く 魂のヴィジョン Stand Proud!
Michi wo hiraku Tamashi no bijon Stand Proud!
闇の中 嗤う背中を
Yami no naka warau senaka wo
探し追い求める男の
Sagashi oimotomeru otoko no
その足音 レクイエム
Sono ashioto Rekuiemu
砂漠に響く…
Sabaku ni hibiku
Stand up! Stand up! Stand up!
Stand up! Stand up! Stand up!
(Stand up! Stand up! Stand up!)
打ち込むのは
Uchikomu no wa
What you're firing
All right now All right now All right now
All right now All right now All right now
All right now All right now All right now
誇りのBullet
Hokori no Bullet
is a bullet of pride
Break you down Break you down Break you down
Break you down Break you down Break you down
(Breakdown! Breakdown! Breakdown!)
拳放つ 聖なるヴィジョン Stand Proud!
kobushi hanatsu Seinaru bijon Stand Proud!
Let its fists fly this hallowed vision, STAND PROUD!
Stand up! Stand up! Stand up!
Stand up! Stand up! Stand up!
(Stand up! Stand up! Stand up!)
立ちはだかる
Tachihadakaru
All right now All right now All right now
All right now All right now All right now
All right now All right now All right now
敵を倒し
Teki wo taoshi
Break you down Break you down Break you down
Break you down Break you down Break you down
(Breakdown! Breakdown! Breakdown!)
道を拓く 魂のヴィジョン Stand Proud!
Michi wo hiraku Tamashi no bijon Stand Proud!
Trivia
The opening has some nods to the events of Part 3, such as having Strength, the cart where Yellow Temperance absorbs the dog and The Sun being in the background behind Joseph, Avdol and Polnareff. Additionally, during the travelling montage, different locations from Part 3 can be seen, such as the fields where the battle with Judgement took place as well as a quick glimpse of what seems to be Hol Horse walking past them.
Due to joining the team only after they finally arrive in Egypt Iggy isn't seen anywhere in the opening, however during the opening there are shooting stars representing the main characters with one being far away from the others most likely being a reference to Iggy not yet joining the team, however its more than likely the opening will be altered to show Iggy once he appears in the anime.