A young woman whom the Aztec Chief and his cult bound on an altar as a sacrifice in a ritual. When the chief plunged a dagger into her chest, her blood splattered all over his mask, activating the mask and piercing its claws into his brain, turning him into a vampire.
A cannibalistic cult that existed during the 12-16th century, led by a Chieftain. They cheer on as the chieftain sacrifices a young woman on an alter for her blood. After his Stone Mask is soaked in her blood and he becomes a vampire, the chieftain drains the blood of one of his cult members in consent.
However, at some point, this tribe disappears from history. It's possible that the chieftain razed the tribe himself.
A woman that appears during the flashback of George Joestar's carriage accident. Dario Brando says that she was a woman he met in a bar. She notices the dead woman and the baby inside the carriage and informs Dario. She is reluctant to go along with Dario's moneymaking schemes of stealing from the victims of the accident, but is soon persuaded. She is often mistaken for being Dio's Mother.
Little is known about her. She was a poor woman married to Dario Brando. She appeared to be one of the few people Dio ever cared for, as he mentions that he hated his father for making her suffer and "working her to death." The date of her death was between 1867 and 1880.
A pair of youths who bully Erina Pendleton with one of them stealing her doll before Jonathan Joestar intervenes. The two recognize Jonathan as a Joestar and proceed to beat him up before walking off. The two later appear as associates of young Dio Brando and were witnesses to him forcefully kissing Erina. One of them expresses an admiration for Dio's bold move.
They are playable characters in the extra battles mode in the Phantom Blood PS2 game.
The announcer of boxing ring matches. Jonathan Joestar was originally going to fight the champion Mark Watkin but the announcer declares there's a change in plans and the new challenger is Dio Brando. The announcer thinks that boxing will be a good way to get to know Dio's personality as they know nothing about him. He explains that boxing is no mere child's game, but Dio accepts the challenge and wagers his entire monthly allowance from George Joestar, surprising the announcer. He explains the rules before the match finally begins.
The former friends of Jonathan Joestar. Jonathan spots them having fun while he's up on a tree and he calls them to come up and smoke a pipe with him. They leer at him spitefully, mentioning to each other that some weirdo is looking at them and that they should head the other way because the place is dangerous since Jonathan would snitch on them. Jonathan jumps down from the tree and punches one of them but the larger kid knocks Jonathan on the back of his head. After they leave, Jonathan realizes Dio must be spreading lies about him.
A butler of the Joestar Family. He works in the Joestar Mansion taking care of George, Jonathan, and Dio. One day while he is taking out the trash, he notices a wooden box inside the incinerator and figures one of the maids left it there. He turns on the incinerator until he hears a loud noise like someone pounding on the door. Realizing his mistake, he immediately opens the door and Danny bursts out completely on fire. The butler notices that someone tied together Danny's maws with wire so he wouldn't be able to bark or bite free. Guilt-ridden, he apologizes to Jonathan and tries to console him once Jonathan comes home to see Danny buried. In the anime, his feelings of guilt were removed as he was replaced with another caretaker during Danny's funeral scene.
The butler later appears to bring George Joestar tea, as well as his medicine afterwards. Dio offers to bring the medicine up to George instead, but Jonathan confronts him asking whether he's poisoned it. Jonathan soon declares that George should only accept medicine from a few trusted doctors who Jonathan brought over. This causes the butler to feel ashamed, crying that Jonathan doesn't trust him and the other caretakers to nurse George to good health. George calms down the butler, saying he doesn't know his son's reasons but he trusts him so they should follow his wish.
Worried that Dio will try poisoning George Joestar again, Jonathan brings these doctors to his father's room and declares that he should only accept treatment from these four doctors and no one else.
After Jonathan leaves to Ogre Street, one of the doctors mentions that JoJo has grown into a reliable young man, having been somewhat unreliable when he was younger. He also states that Jonathan's features have changed him well. George mentions that is why he would often get more angry at Jonathan than necessary as the discipline benefited him. This scene is omitted from the anime.
After Jonathan Joestar discovers that Dio poisoned his father and sets off to find a cure, Dio becomes paranoid and wanders through the streets whilst drinking. He eventually bumps into a tramp and his friend and picks a fight with them. Dio decides to use one of them as a live test subject for the Stone Mask, believing it to be an instrument of torture and murder. He then forces one of the vagrants to put on the mask and stabs his partner in the throat. The mask, now soaked in blood, turns the man into a vampire. He attacks Dio and becomes younger while absorbing Dio's blood, but disintegrates after witnessing the sunlight.
A policeman at the Joestar Mansion who is killed by Dio Brando. Dio impales through the man's helmet and pierces the man's head using only his fingers. After draining him completely of his blood, the corpse returns desiring blood, becoming the first zombie created by Dio. The zombie attempts to drink Speedwagon's blood and grabs his face. However, his meal is interrupted by Jonathan who uses a spear to decapitate him. Despite being a monster, Jonathan feels pain from killing the man and asks for strength from his late father.
He is a playable character in the extra battles mode in the Phantom Blood PS2 game.
In Whitechapel, London, this woman is with her boyfriend and notices that it is already 11:45 PM. Realizing how late it is, she says they better hurry home. However, the man says it's still early and they should go somewhere else instead. She refuses, worried that Jack the Ripper will appear since he's been in the newspapers lately. He only targets women and uses a surgical scalpel, going as far as to hang the intestines of his victims up on a wall. Smirking, her date reveals that she shouldn't be terrified because she was talking to Jack the Ripper the whole time. Stunned when seeing his scalpel, Jack mentions that she was out playing too late and decapitates her before she could react.
In the anime, her dialogue is omitted and only her scream and a glimpse of her body on the ground when being killed by Jack is shown.
A fisherman that Will A. Zeppeli met on the Caribbean when he was young. The ocean where he lived was a deadly trap, with fearsome sharks over ten meters tall that killed several men from his village. When the man went out to fish, the sharks attacked his small boat, causing him to slip and fall into the water. To save himself, he cut off his leg with the blade of his harpoon. However, instead of escaping, he called a shark to him with the blood of his wound. As the shark approached him, he stabbed it in the head with the blade of his harpoon. His victory resulted in him becoming a hero of the village and he lived out his life in happiness. Zeppeli refers to his bravery as the birth of a viking.
He is omitted from the anime, with only Zeppeli's dialogue about vikings remaining.
After Poco is hypnotized by Dio to lure Jonathan, Zeppeli, and Speedwagon to the graveyard of Windknight's Lot, they are attacked by several zombie knights who appear out of the ground. Zeppeli uses his own version of Sunlight Yellow Overdrive to karate chop one of them in the face, simultaneously killing three more when the body is sent flying into the zombies behind it. Speedwagon crushes one in the head using his sledgehammer. Jonathan decapitates the final one with Zoom Punch.
In the anime, Speedwagon killing one of them with his sledgehammer is omitted.
An executioner working for Elizabeth I. Before executing Tarkus and Bruford, he gives them a "present", pointing to Mary's decapitated head on the ground. He boasts about being the one to kill her and mentions that the woman imprisoned in the tower was just an impersonator to trick the knights. They thought letting her live would risk the chance of a rebellion. Enraged, Bruford and Tarkus screamed and cursed Elizabeth and all her descendants. Rigid from anger, Tarkus broke several axes of the executioners with the flesh of his thick neck, before fully giving way. Bruford's long hair supposedly entangled the feet of the executioner and tore his flesh before dying.
Two adult bullies of Poco drag him back to the graveyard in Windknight's Lot after he tells them he saw ghosts of the dead knights. The bald man says he won't be fooled anymore because he believed Poco last time he was told that rabbit droppings were good for stomachaches. His friend threatens to tie Poco up and have him swing over the graveyard by a rope. As Poco cries out that they'll kill him, the bald man tells him to shut up and punches him. As they laugh at how scared he is, one of them steps on something strangely "elastic" before recognizing a face. Frightened at the mangled faces of corpses mashed together, they realize Poco was telling the truth. They are immediately greeted by Tarkus and flee, leaving Poco to die by the "monster". Jonathan saves Poco, but Tarkus grabs the two bullies by their feet. He crushes them with his grip and wrings them together like laundry until their flesh comes off, drinking their blood as it lands in his mouth. The anime omits these two bullies.
Later, Poco recalls being bullied by teenagers who tease him and push him around for not looking at their faces. In the manga, one even tries to burn him with his cigarette. When Poco's Sister shows up to confront them, they flee out of fear.
A young man who calls himself a doctor. He uses the Ripple to heal the diseases and injuries of his patients. When Zeppeli finds him in India, he is healing an old man using the Ripple by simply touching the patient's leg. Western medicine would have had to amputate the leg to save his life, but the doctor instantly turns the man's skin pink again, informing him that he should be able to walk again in 10 days. Zeppeli asks how the doctor got his powers and he tells him to go up the Salween River and look for his master, Tonpetty.
Men that couldn't stand the terror of Dio and sacrificed their souls to him. Dio uses them as an example when trying to convince a mother that it's better to join them and be like a family. They stay behind Dio, standing on the ceiling while the woman looks at them in fear.
A woman who pleads with Dio to spare her baby. Dio promises that he and his men will not harm her infant. He turns the lady into a zombie and she immediately eats her child while it cries. Dio calls it a tragedy that she chose to do herself.
Mutated creatures created by Dio Brando as a result of experimenting with his prey. He mixed the heads and bodies of various humans and animals. One of the creatures interrupts while Dio is trying to bring Poco's Sister to his side, saying that she's young and full of life energy so she must be tasty. Angered due to the creature lacking etiquette, Dio kicks the chimera into the air and stomps on it, killing it.
When Poco returns home, he finds his father, who slaps him and asks him where he had been. Poco doesn't answer the question and instead asks his father where his sister is. His father replies that she went to look for him.
Zombies who are loyal to Dio for giving them freedom after their executions. A few of their equipment resemble the armor of Tarkus and Bruford. One of them asks for Dio to give them the order to execute Jonathan, as another says he'll rip him apart with his fangs. Before Dio could respond, one of the zombies hiding on the ceiling above Jonathan charges at him with his claws. However, Jonathan easily dodges without looking back and slams the zombie's head into prison bars, with enough force to slice its head open. Dio tells them Jonathan is only his opponent, so they attack Straizo and Tonpetty later instead. The Ripple warriors kill them off-screen.
A man working at a bar in London near the ship docking area. Speedwagon was sitting at his bar reading the London Press newspaper when he lost track of time, realizing Jonathan and Erina were already about to leave on their ship to America. He rushes out of the bar without paying, causing the bartender to chase after him. He finally grabs onto Speedwagon and yells at him to pay for his meal.
Three men carrying a large coffin who accidentally bump into Speedwagon on their way to the ship that Jonathan and Erina were going to be on. They apologize saying they have to hurry and get to the ship before it leaves. The men wonder why it's so heavy, but all they know was that some weird oriental guy paid a lot of money for them to bring it onto the ship. One of the men hears a quiet "WRYYY" from Dio and realizes something is inside it but the man behind him tells him to stop talking and to just load it on the ship before it leaves.
By the command of Dio, Wang Chan feasts on the blood of a single crewman on the ship headed to America, and eventually the entire ship becomes filled with Zombies. They kill every human passenger on the ship aside from Erina, Jonathan, and a baby. When Jonathan uses his final Ripple to control Wang Chan into grabbing onto the ship's screw shaft, Dio orders the zombies to devour Wang Chan's body. However, they are immediately engulfed by the ship's explosion and burn to ashes.
The mother of a baby who would later be named Elizabeth Joestar. She and her husband are on the same ship as Jonathan and Erina but a zombie attacks her with an axe and she falls down the stairs, dying while carrying her baby in her arms. Although it is unclear who her husband could be, he was murdered on the ship by zombies as well. As the baby cries, Jonathan tells Erina to get off the ship without him while rescuing the baby. She reminded him of his own mother who died while holding him in her arms.