Multiple different versions of Dio appear in the game, reflecting the events of the original story. Although two iterations can be used in 77 Rings Knights Mode, most of his playable variations are exclusive to Dio Mode, a mode where the player follows the story through Dio's perspective.
After defeating the undead Bruford and Tarkus in the tenth Dio Mode fight, Dio faces the three 77 Rings champions in the eleventh, followed by Doobie and Dio's own zombie chimeras in the twelfth; Zeppeli and a hammer-wielding Speedwagon confront Dio in the thirteenth fight, and the three Ripple Warriors fight Dio in the fourteenth. The fifteenth stage of Dio Mode sees Dio engaging in a climactic battle with Jonathan, who is accompanied by Speedwagon. This form of Dio can also be unlocked in 77 Rings Knights Mode.
In the secret alternate version of Chapter 23, Dio grafts his head onto Father Styx's body during his final battle with Jonathan; this version of Dio is identical to his previous form moveset-wise, and is non-playable.
- 77 Rings Knights Mode: Living Corpses
- 77 Rings Knights Mode: Living Corpses