Eyes of Heaven/Yoshikage Kira

"I can't let a single person that knows the truth of "Yoshikage Kira" live another day... (誰ひとりとしてこの『吉良吉影」の正体を知る者はいてはいけないんだよ・・・)"

Yoshikage Kira (吉良 吉影) was confirmed for the game alongside,. As a Stand user, Kira is one of many characters with wildly varied abilities that grant him uniqueness in battle. Most of his animations and moveset were reused from the previous game.

Moveset

 * Style Action - The First Bomb!: The action performed is determined by whether or not there is a bomb active. If an object or opponent is currently a bomb, Killer Queen detonates them, inflicting moderate damage in a small area, and doing more damage to the primed target. An opponent can even be detonated when downed, provided they are not in an invulnerable state. If there is no bomb active, Killer Queen strikes a pose, and then performs an unblockable downward chop, dealing medium damage to an opponent while also charging them as an unblockable bomb. Only one thing can be charged as a bomb at any given time, so if a bomb is already active, certain attacks that release or charge bombs will not do so. Sheer Heart Attack does not influence this rule. Also, instead of throwing or breaking larger stage objects, Killer Queen is able to charge them as contact bombs that can also be detonated at will; Kira cannot perform this if a bomb is already active.
 * Bomb Transformation Icon: A small Killer Queen skull icon on the right side of Kira's health bar shows whether or not he currently has a bomb charged. If it is darkened, no bomb is active.
 * Trait - Perfect! Nobody can stop me!: If Kira takes no damage within the first 15 counts of the battle timer, he temporarily gains extra defense, as well as increased cooldown speed and stamina regeneration.
 * Sheer Heart Attack: Killer Queen sends out its second bomb, Sheer Heart Attack to blow up enemies. SHA will home in on a target, and deals damage to anything in a small area, including Kira and/or his partner, when it detonates. It leaps at its target when it attacks, and successful or not, it will then be left overturned and lay harmless on the ground for a few seconds. It will detonate as many times as it can before its duration expires and it's forced to return to Killer Queen, which also interrupts Kira. Notably, Sheer Heart Attack homes onto the hottest thing in the area, and its explosion size is proportional to the heat (e.g. characters like and  have fire-based attacks that will become SHA's priority, causing a larger explosion and dealing more damage). The same applies to the Joestar Mansion map when it begins to burn down, and Echoes ACT2's "sizzle" SFX at Boing-Boing Cape. SHA has unique interactions with other characters.
 * Similar to All-Star Battle, if Koichi's Echoes ACT3 performs any skill that inflicts the 3 Freeze effect ("Heavy"), SHA will be weighed down and temporarily unable to move.
 * If SHA comes into contact with the erased space generated by Okuyasu Nijimura's The Hand, it will be erased itself, and Kira will take a medium amount of damage. SHA can still be reused after this.
 * You bastard!: Killer Queen unleashes a barrage of 12 punches, sending opponents caught flying on the final hit. If no bomb is active, the final hit also charges the target as one. This ability can be overpowered by any other close-ranged Stand.
 * Anything I touch...turns into a bomb.: Killer Queen flicks forward a coin charged with the first bomb, bouncing a few times and indicated by a small shine on the ground. The coin can act as a projectile that deals small damage until it stops moving, and can also only be detonated when it does so. If a bomb is already active, the coin is unable to be detonated and remains a simple, arcing projectile.
 * Killer Queen: Killer Queen strikes a defensive stance by rapidly moving its hands around its face, blocking any non-projectile, non-DHA attack. If an opponent triggers the counter, Kira Sidesteps with invincibility, avoiding damage. This also has the effect of filling the Dual Heat Gauge by a considerable amount.
 * The in-game menu description erroneously describes the skill as being able to turn opponents that trigger the counter into bombs, as well as having an EX version that "activates quicker and uses an enhanced bomb" despite it not being among Kira's two EX skills. This could be describing an earlier version of the skill that was changed to its current form before the game's release, and was mistakenly left in.
 * EX - You Bastard!: This skill executes quicker, and the bomb charged on the final hit creates a larger explosion, dealing more damage and having more range.
 * EX - Anything I touch...turns into a bomb.: Kira can act faster out of the skill, and the bomb charged creates a larger explosion, dealing more damage and having more range.
 * Dual Heat Attack - I'm going to blow you to smithereens!: Killer Queen punches the opponent to the floor, before Kira and it strike a pose. Charging its power, the Stand chops the downed opponent, turning them into a bomb, then Kira takes a step back and annihilates them with a single, massive explosion.

JoJolities

 * I'm going to blow you to smithereens!: Kira must connect his Style Action's attack 3 times. (200 Points)
 * Killer Queen has already touched the door knob...: Kira must charge a stage object as a bomb 3 times. (200 Points)
 * All I want is to live peacefully.: Kira must survive the first 20 counts of the battle timer without taking damage. (300 Points)
 * I never fail to eliminate my targets...: Kira must Retire an opponent with "Sheer Heart Attack". (500 Points)
 * Tonight...I'm going to sleep better than I have in a long time...: Kira must Retire an opponent without losing a single health gauge. (800 Points)

If Kira is matched with Kosaku-Kira, the latter will comment on how he's on Kira's side, only for Kira to wonder how Kosaku-Kira knows his identity. Kira also has special interactions with all the Part 4 protagonists.

In the game's altered timeline the protagonists managed to defeat Kira before he could kill Shigechi, the real Kosaku Kawajiri and presumably Aya.

Tournament
He is paired with in the Eyes of Heaven tournament, defeating  and  in the first round. They were eliminated by and  in the second round, whom beforehand, won the F Block series of battles that allowed them to be matched against Kira and Old Joseph within the main tournament.