Eyes of Heaven/Jonathan Joestar

Jonathan Joestar was confirmed for the game alongside Will Anthonio Zeppeli and Robert E. O. Speedwagon. Unlike the previous game.

Jonathan's main color scheme is now based on his anime appearance. Additionally, Kazuyuki Okitsu reprises his voice-acting role from All-Star Battle, having also voiced Jonathan in the Phantom Blood anime adaptation.

Moveset
Most of his moveset and character model remain the same from All-Star Battle. New additions to Jonathan's attacks include him using the spear he attacked Dio with shortly after his transformation as a Powerful Attack. Several of his new combos and sidesteps are now based on his boxing skills (displayed during his childhood in Part 1).

As a Ripple user, Jonathan has a Ripple Gauge that determines the strength of his attacks.

JoJolities

 * One-in-a-Million Potential: Jonathan must completely fill his Ripple Gauge. (200 Points)
 * Father! Grant me your final power!: Jonathan must connect a Powerful Attack as the third step in a mid-air combo. (200 Points)
 * I want to become a true gentleman!: Jonathan must successfully execute 5 Combo Breakers. (300 Points)
 * I'll knock you until you cry like a little girl!: Jonathan must achieve a collective hit count of 200. (500 Points)
 * Own your fear!: Jonathan must Retire an enemy with a Dual Heal Attack. (800 Points)

Tournament
He is paired with DIO in the Eyes of Heaven Tournament, defeating Baron Zeppeli and Koichi Hirose in the first round and Wamuu and N'Doul in the second. In the semifinals, they defeated Mariah and Esidisi in the first round, but were eliminated by Kars and Pet Shop in the second.

Relationships in Eyes of Heaven
(The information below derives from a Video Game not written by Araki. As such, it may not be considered canon.)
 * Giorno Giovanna: When the two are on the same team, Giorno will ask Jonathan everything he knows about DIO. If victorious, Giorno will mention that DIO is his father, surprising Jonathan.
 * Jotaro Kujo: At first it was upsetting that Jotaro referred to Jonathan as his ancestor. Later Jonathan felt in his heart that he was succeeding his own lineage, to the point of asking Jotaro to finish Heaven Ascension DIO in his place during the main story's final battle. Also, if Jotaro is Part 4 version, he mentioned to Jonathan's grandson and Jotaro's grandfather Joseph Joestar.
 * Joseph Joestar: Jonathan is impressed by Joseph's tactics and Ripple. He states that Old Joseph reminds him of his father.
 * Kars: Upon encountering him, Jonathan will recognize Kars as the one who created the Stone Masks and seeks to kill him and destroy the Stone Masks. Kars seeks to kill Jonathan simply because he is a Hamon user and later attempts to recruit him to his side, considering his death to be a waste.