Agents of DIO

DIO's organisation is the primary antagonistic force in the original universe of JoJo's Bizarre Adventure, with its members appearing in Phantom Blood, Battle Tendency, Stardust Crusaders, Diamond Is Unbreakable and Stone Ocean.

Description
The group was formed by Dio Brando, through sheer charisma and due to his various desires of dominating the world, finding "Heaven", and destroying the Joestar Group. Even after DIO's death, the remnants of his group appear in later parts. In Part 6, DIO's followers serve as major antagonists continuing his legacy.

Phantom Blood
Dio meets Wang Chan who becomes his first loyal subordinate. He then creates an army of Vampires and Zombies under his control including Jack the Ripper, Bruford, Tarkus, Doobie, Adams, Page, Jones, Plant, and Bornnam.

Battle Tendency
With Dio supposedly dead after the events of Phantom Blood and his followers thought to be killed by Jonathan Joestar and his allies, Dio's group was disbanded. However, there were some minions that survived, including a bloodsucking zombie which disguised itself as a major squad leader in the. He was later killed by Lisa Lisa as revenge for George Joestar II's murder.

Stardust Crusaders
Nearly a hundred years later, DIO resurfaces and obtains a Stand from Enya the Hag. With Enya as his adviser, DIO forms a new group mainly consisting of human Stand users and only a few vampires. In secret, he befriends an Italian priest named Enrico Pucci, whom he names his successor in the event of his death. He recruits members from all around the world, primarily mercenaries and manipulates many of them with large sums of money like Hol Horse. For more hesitant or untrustworthy followers, DIO uses flesh buds to enthrall them, such as Noriaki Kakyoin and Jean Pierre Polnareff.

Although DIO fails to achieve his goal and the majority of his group is defeated once again, he and Vanilla Ice succeed in killing half of the Joestar group before their deaths.

Diamond is Unbreakable
Yoshihiro Kira and Nijimura's Father were recruited by DIO in the past and both retrieved a Bow and Arrow from Enya. As a result, many citizens of Morioh gained Stands, including the sons of the aforementioned.

After Dio's death, Nijimura's father suffered a horrific transformation that left him unable to die. Yoshihiro defected and turned his attention to helping his son achieve his goals instead.

Stone Ocean
The final remaining servants of DIO's group are Enrico Pucci and Johngalli A with the goal of avenging DIO by killing Jotaro Kujo and his daughter, Jolyne Cujoh. Pucci desires to continue DIO's dream of finding Heaven and recruits DIO's sons as well as the prison staff and inmates to help him defeat the Joestar Group.

Pucci does succeed in killing the Joestars and their allies, and almost manages to reshape the universe in DIO's image. However, Emporio kills him before he is able to do so, making the world free of DIO's influence. In the alternate universe, the deceased Joestars and their friends are reborn as alternative counterparts, with different lives and memories.