Eyes of Heaven/Dio Brando

Dio Brando (ディオ・ブランドー) as he appears in Phantom Blood was confirmed for Eyes of Heaven alongside, and.

He is of the Vampire style. As such, a number of his abilities allow him to not only drain his opponents' health, but also to enter a Vampiric Power-Up Mode, boosting his movement speed and attack damage, among other things. All damage to him aside from Ripple-based will become "white damage", that can be healed over time, and inversely, Dio will take more damage from Ripple-based attacks.

With the release of the Phantom Blood anime, his costume is completely different to that of All-Star Battle, replacing his primarily yellow color-scheme with a redder look. Additionally, Takehito Koyasu reprises his voice acting role from All-Star Battle, having also voiced Dio in the anime.

JoJolities

 * Prepare to be frozen in your tracks!: Dio must connect "MUDA MUDA MUDA!" 3 times. (200 Points)
 * Do you remember how many breads you have eaten in your life?: Dio must connect his Style Action 5 times. (200 Points)
 * I, Dio, will kill you!: Dio must Retire an opponent. (300 Points)
 * I reject my humanity!: Dio must retain a Vampiric Power-Up Mode for 20 counts of the battle timer. (500 Points)
 * You thought you stood a chance?: Dio must win within 50 counts of the battle timer. (800 Points)

Tournament
Dio Brando is paired with in the Eyes of Heaven Tournament, defeating  and  in the first round, but eliminated by  and  in the second.