Yuki Ono was born in Shizuoka Prefecture, Japan, in 1984. Ono initially wanted to have a career in football, and was even scouted by the Shimizu S-Pulse local football club. However, he suffered from a bone fracture.
During his years in university, Ono learned about voice actor Kishō Taniyama. He became a fan of Taniyama's work and after a meeting with him, was inspired into becoming a voice actor. Ono started a career in voice acting. In August 2011, Ono formed the voice acting unit You-Tak alongside voice actor Takuya Eguchi., which was soon rebranded as "You-Tak II". In March 2013, he was awarded for "best actors in supporting roles" in the 7th Seiyu Awards.
On October 2, 2017, Ono announced his marriage. On August 2020, Ono announced the birth of his first child.